Laboratory

The Rainey Labs are located in the Interdisciplinary Research Facility (CA building) on the MCG campus.

The research building lab facilities have been designed and equipped to facilitate state-of-the-art research and learning.  New technologies, techniques, and equipment are constantly being evaluated and integrated into the lab protocols as appropriate – such as GeneSpring Microarray Data analysis, real time PCR, and immunohistochemistry.

The Physiology Department at MCG host visiting scientists on a continuing basis, with lectures every Thursday. Students, Post Docs and Professors participate with questions and discussions.  These lectures/discussions are held in the newly constructed seminar/multi-media facilities in the CA building.

 

Numerous other group discussion areas are used for casual meetings, presentations and lab meetings.

 

 

 

Facilities & Equipment

The research facilities consist of two lab rooms (CA3091 and CA3093).  Each room is 1,200 sq ft and consists of general research benches, tissue culture lab and a steroid analysis room.

Equipment – CA 3091 & CA 3093

The Rainey Research Lab uses a variety of techniques and technologies which often necessitates the use of precision-engineered equipment. The equipment plays an important role in investigation in almost every stage of research and scientific analysis. The extensive equipment in the lab allows for advancement of science without ever leaving the laboratory.

Techniques such as PCR, DNA subcloning, gene manipulations, DNA and RNA preparation and purification, lenti-virus construct development, and immunohistochemistry are routine procedures of the laboratory. The laboratory equipment is key in allowing for high through-put, efficient, and accurate performance of these essential protocols.

Steroid Analysis Core Lab Equipment:
Elisa Plate Reader, Washer, Shaker
Reacti-Therm III Heating Module - Drying GC Samples
Gas Chromatography Mass Spectrometry (GS/MS) System
Rotating Shaker (open) and Steroid Drying Apparatus
Liquid Scintillation Counter
RNA Isolation and Analysis Equipment:
Gel Drying System
Standard Spectrophotometers
ABI Real-Time RTPCR Apparatus and RTPCR Sterile Hood
NanoDrop RNA Quantification Spectrophotometer
PCR Thermocycler
Phosphoimager
DNA Isolation, Manipulation and Analysis Equipment:
eppendorf - mini spinners
Low speed, high speed and ultra centrifuges
Bacterial incubator Shaker (closed)
Gel Documentation System
Bacterial Plate Incubator
Cell Culture Core Lab Equipment:
Biosafety Containment Hoods
Tissue Culture Incubators
Inverted Fluorescence Microscope
Coulter Cell Counter
Liquid Nitrogen Cell Storage Unit
Tissue Culture Microscope
General Core Lab Equipment:
Milli-Q Water Purification System
Balances - Top Loading, Metler

Key Protocols

Some selected “key protocols” are included below.

          Tissue Culture

-  H295 Medium Preparation
-  Subculture Procedure
-  Experiment Setup on 12 Well Culture Dishes
-  Transfections
-  Cell Cloning

          Real Time

-  Primers for Real Time PCR
-  Reverse Transcription
-  Reverse TranscriptioReal Time PCR