Our research focuses on the control of renal hemodynamics at the microcirculatory level. Physiological and pharmacological influences on renal vascular resistance vessels are investigated using up-to-date videomicroscopy techniques that provide direct access to pre- and post-glomerular microvasculature. Application of these techniques permits direct assessment of renal microvascular function and microvascular responses to selected experimental manipulations. Of particular interest is the role of renal P2 receptors in controlling pre- and post-glomerular resistance and their effect on renal hemodynamics and autoregulatory control. We also examine the impact of hypertension, diabetes and inflammation on renal function, renal microvascular function and renal injury. In conjunction with these studies, we investigate the calcium signal transduction pathways utilized by vasoactive agents to modulate renal microvascular resistance. Experiments are performed using intact renal microvascular segments as well as with single vascular smooth muscle cells isolated from preglomerular microvascular elements. The ability to define intracellular responses such as ion fluxes or second messenger systems in vascular smooth muscle cells may permit us to better explain the behavior of renal microvessels in an intact system and offer clues toward explaining abnormal function in disease states like hypertension and diabetes.
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Schneider, Markus P., Edward W. Inscho and David M. Pollock. Attenuated vasoconstrictor responses to endothelin in afferent arterioles during a high salt diet. American Journal of Physiology: Renal Physiology, 292:F1208-14, 2007.
Rivera, Ian, Shali Zhang, Barry Fuller, Brentan Edwards, Mario B. Marrero and Edward W. Inscho. P2 receptor regulation of [Ca2+]i in cultured mouse mesangial cells. American Journal of Physiology-Renal Physiology: 292: F1380-F1389, 2007.
Rita C. Tostes, Fernanda R.C. Giachini1, Fernando S. Carneiro, Romulo Leite, Edward W. Inscho and R. Clinton Webb. Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. J. Pharmacol. Exp. Therapeutics. 322: 768-685, 2007
Guan, Zhengrong, David A. Osmond and Edward W. Inscho. Purinoceptors in the kidney. Experimental Biology and Medicine, 232 (6): 715-726, 2007.
Inscho, Edward W., John D. Imig, Anthony K. Cook, David M. Pollock. ETA and ETB Receptors Differentially Modulate Afferent and Efferent Arteriolar Responses to Endothelin. British Journal of Pharmacology, 146: 1019-1026, 2005.
Zhao, Xueying, Anthony K. Cook, Mary Field, Brentan Edwards, Shali Zhang, Zhanying Zhang, Jennifer S. Pollock, John D. Imig and Edward W. Inscho. Calcium signaling is involved in attenuated purinoceptor-mediated vasoconstriction of afferent arterioles in angiotensin II-induced hypertension. Hypertension, 46: 562-568, 2005.
Sharma, Kumar, Anthony K. Cook, Matt Smith and Edward W. Inscho. TGF-beta impairs renal autoregulation via ROS generation. American Journal of Physiology Renal Physiology 288: F1069-F1077, 2005.
Pollock, David M., John M. Jenkins, Anthony K. Cook, John D. Imig and Edward W. Inscho. L-type calcium channels in the renal microcirculatory response to endothelin. American Journal of Physiology: Renal Physiology 288: F771-F777, 2005.
Nishiyama, Akira and Edward W. Inscho. Role of adenosine and ATP in the regulation of renal hemodynamics and microvascular function. Hypertension Research 27: 791-804, 2004.
Inscho, Edward W., Anthony K. Cook, Jim Murzynowski and John D. Imig. Elevated arterial pressure impairs autoregulation independently of AT1 receptor activation. Journal of Hypertension 22: 811-818, 2004.
Inscho, Edward W., Anthony K. Cook, John D. Imig, Catherine Vial and Richard J. Evans. Physiological Role for P2X1 Receptors in Renal Microvascular Autoregulatory Behavior. The Journal of Clinical Investigation 112: 1895-1905, 2003.
