Medical College of Georgia :: Department of Pharmacology & Toxicology

		Nevin Alan Lambert, Ph.D., Associate Professor Phone: (706) 721-6336 Fax: (706) 721-2347 Email: nlambert@mail.mcg.edu Office: CB-3522 Grant Support \| Current Projects \| Lab \| Honors & Awards \| Invited Speaker \| Selected Publications \|
Research Interests Grant Support Active: Regulation of G-protein signaling in CNS neurons, R01NS36455, 4/97-1/07. Total direct cost: $665,000 (budget period 2/03-1/07). Calcium and Endocytotic Membrane Retrieval, R01NS41055, 4/00-3/05. Total direct cost: $800,000. Current Projects Lab Graduate Student Supervision (as major advisor): 1996-2001 Huanmian Chen (Ph.D. 2001) 1999-2003 Jonathon Wetherington (Ph.D. 2003) 2002-2003 Chadwick Hales (Ph.D. 2003; co-advisor with J. Goldenring) 2001- Michael Clark 2002- Robert Lober 2004- Gregory Digby Postdoctoral Fellows: 1997 Caroline E. Rick, Ph.D., (visiting fellow, University of Chicago) 1997-2001 Xueyong Wang, Ph.D. 2000-2004 Rebecca Brogan, Ph.D. Honors and Awards Stephen W. Kuffler Fellow, Marine Biological Laboratory, 2003. Summer Research Fellowship, National Institutes of Health, 1987. Kent State University Fellowship, 1991. Graduate Student Research Award, 1991. Invited Speaker Institut de Cardiologie de Montreal, University of Montreal, Quebec, Canada, 9/05. Gordon Conference on Molecular Pharmacology, Il Ciocco, Italy, 5/05. Georgia Institute of Technology, Coulter Department of Biomedical Engineering, 4/05. Albert Einstein University College of Medicine, Department of Neurobiology, 12/04. University of Alabama Birmingham, Department of Physiology and Biophysics, 12/03. University of Georgia, Neuroscience, 4/02. Boston University, Department of Biology, 4/02. Guthrie Research Institute, Sayre, PA 8/01. University of Colorado Health Sciences Center, 9/00. University of South Carolina College of Medicine, Dept. of Cell Biology and Anatomy, 5/97. Marshall University School of Medicine, Department of Physiology, 5/97. Selected Publications Clark, M.A. and Lambert, N.A. Endogenous RGS Proteins Regulate the Kinetics of Gaq/11-Mediated Modulation of Ion Channels in CNS Neurons. Molecular Pharmacology, 69:1280-7, 2006. Schulz, T.C., Noggle, S.A., Palmarini, G.M., Weiler, D.A., Lyons, I.G., Pensa, K.A., Meedeniya, A.C.B., Davidson, B.P., Lambert, N.A., Condie, B.G. Differentiation of Human Embryonic Stem Cells to Dopaminergic Neurons in Serum Free Suspension Culture. Stem Cells, 22:1218-38, 2004. Chen, H., Clark, M.A. and Lambert, N.A. Endogenous RGS proteins regulate presynaptic and postsynaptic function: functional expression of RGS-insensitive Ga subunits in CNS neurons. Methods in Enzymology, 389:190-204, 2004 (invited). Calhoun, J.D., Lambert, N.A., Mitalipova, M.M., Noggle, S.A., Lyons, I. Condie, B.G., Stice, S.L. Differentiation of Rhesus ES Cells to Neurons and Neural Progenitors. Biochem. Biophys. Res. Commun., 306:191-7, 2003. Wang, X. and Lambert, N.A. Membrane Properties of Identified Lateral and Medial Perforant Pathway Projection Neurons. Neuroscience, 117:485-492, 2003. Wetherington, J.P. and Lambert, N.A. GABAB receptor activation desensitizes postsynaptic GABAB and A1 adenosine responses in rat hippocampal neurons. J. Physiol., 544:459-467, 2002. Smith, R.M., Baibakov, B., Lambert, N.A., and Vogel, S.S. Low pH Inhibits Compensatory Endocytosis at a Step Between Depolarization and Calcium Influx. Traffic. 3:397-406, 2002. Wetherington, J.P. and Lambert, N.A. Differential desensitization of responses mediated by presynaptic and postsynaptic A1 adenosine receptors. J. Neurosci., 22:1248-55, 2002. Koushik, S.V., Wang, J., Rogers, R., Moskofidis, D., Lambert, N., Creazzo, T. and Conway, S.J. Targeted inactivation of the sodium-calcium exchanger (Ncx1) results in the lack of a heartbeat and abnormal myofibrillar organization. FASEB J., 15(7):1209-11, 2001. Chen, H. and Lambert, N.A. Endogenous RGS proteins regulate presynaptic inhibition at rat hippocampal synapses. Proc. Natl. Acad. Sci. USA, 97:12810-12815, 2000. Hancock, C.R., Wetherington, J.P., Lambert, N.A. and Condie, B.G. Neuronal differentiation of cryopreserved neural progenitor cells derived from mouse embryonic stem cells. Biochem. Biophys. Res. Commun., 271(2):418-421, 2000. Smith, R.M., Baibakov, B., Ikebuchi, Y., White, B.H., Lambert, N.A., Kaczmarek, L.K. and Vogel S.S., Exocytotic insertion of calcium channels constrains compensatory endocytosis to sites of exocytosis, J. Cell Biol., 148:755-768, 2000. Wang, X. and Lambert, N.A. GABAB receptors couple to potassium and calcium channels on identified lateral perforant pathway projection neurons, J. Neurophysiol., 83:1073-1078, 2000. Vogel, S.S., Smith, R.M., Baibakov, B., Ikebuchi, Y. and Lambert, N.A. Calcium influx is required for endocytotic membrane retrieval, Proc. Natl. Acad. Sci. USA, 96:5019-5024, 1999. Chen, H. and Lambert, N.A. Inhibition of dendritic calcium influx by activation of G-protein-coupled receptors in the hippocampus, J. Neurophysiol., 78:3484?3488, 1997. Lambert, N.A. and Wilson, W.A. High-threshold Ca2+ currents in hippocampal interneurones and their selective inhibition by activation of GABAB receptors. J. Physiol., 492.1:115-127, 1996. Lambert, N. and Grover, L. The mechanism of biphasic GABA responses. Science, 269:928-929, 1995 (invited Perspective). Barr, D.S., Lambert, N.A., Hoyt, K.L., Moore, S.D. and Wilson, W.A. Induction and reversal of long-term potentiation by low- and high-intensity theta-pattern stimulation. J. Neurosci., 15:5402-5410, 1995. Lambert, N.A. and Wilson, W.A. Temporally distinct mechanisms of use-dependent depression at inhibitory synapses in the hippocampus in vitro. J. Neurophysiol., 72:121-130, 1994. Lambert, N.A. and Wilson, W.A. Heterogeneity in presynaptic regulation of GABA release from hippocampal inhibitory neurons. Neuron, 11:1057-1067, 1993. Grover, L.M., Lambert, N.A., Schwartzkroin, P.A. and Teyler, T.J. Role of HCO3- ions in depolarizing GABAA receptor mediated responses in pyramidal cells of rat hippocampus. J. Neurophysiol., 69:1541-1555, 1993. Lambert, N.A. and Wilson, W.A. Discrimination of post- and presynaptic GABAB receptor-mediated responses by tetrahydroaminoacridine in area CA3 of the rat hippocampus. J. Neurophysiol., 69:630-635, 1993. Lovinger, D.L. and Lambert, N.A., Presynaptic glutamate receptors in the mammalian brain, in Presynaptic Receptors in the Mammalian Brain, Dunwiddie, T.V. and Lovinger, D.L. eds., Birkh�user, pp. 127-142, 1993 (invited). Lambert, N.A. and Harrison, N.L., Presynaptic GABAB receptors on inhibitory neurons in the hippocampus, in Presynaptic Receptors in the Mammalian Brain, Dunwiddie, T.V. and Lovinger, D.L. eds., Birkh�user, pp. 143-160, 1993 (invited). Lovinger, D.M., Harrison, N.L. and Lambert, N.A. The actions of 3-aminopropanephosphinic acid (APPA) at GABAB receptors in the rat hippocampus. Eur. J. Pharmacol., 211:337-341, 1992. Lambert, N.A., Levitin, M. and Harrison, N.L. Induction of giant depolarizing potentials by zinc in area CA1 of the rat hippocampus does not result from blockade of GABAB receptors. Neurosci. Lett., 135:215-218, 1992. Lambert, N.A., Borroni, A.M., Grover, L.M. and Teyler, T.J. Hyperpolarizing and depolarizing GABAA receptor-mediated dendritic inhibition in area CA1 of rat hippocampus. J. Neurophysiol., 66:1538-1548, 1991. Lambert, N.A., Harrison, N.L. and Teyler, T.J. Evidence for m opiate receptors on inhibitory terminals in area CA1 of rat hippocampus. Neurosci. Lett., 124:101?104, 1991. Lambert, N.A. and Teyler, T.J. Adenosine depresses excitatory but not fast inhibitory synaptic transmission in area CA1 of the rat hippocampus. Neurosci. Lett., 122:50?52, 1991. Lambert, N.A., Harrison, N.L. and Teyler, T.J. Baclofen?induced disinhibition in area CA1 of the rat hippocampus is resistant to extracellular Ba2+. Brain Res., 547:349-352, 1991. Lambert, N.A. and Teyler, T.J. Cholinergic disinhibition in area CA1 of the rat hippocampus is not mediated by receptors located on inhibitory neurons. Eur. J. Pharmacol., 203:129-131, 1991. Harrison, N.L., Lovinger, D.M., Lambert, N.A., Kerr, D.I.B. and Teyler, T.J. The actions of 2?hydroxy?saclofen at presynaptic GABAB receptors on hippocampal neurons. Neurosci. Lett., 119:272?276, 1990. Lambert, N.A. and Harrison, N.L. Analogs of cyclic AMP decrease gamma?aminobutyric AcidA receptor?mediated chloride current in cultured rat hippocampal neurons via an extracellular site. J. Pharmacol. Exp. Thera., 255:90?94, 1990. Harrison, N.L., Lambert, N.A. and Lovinger, D.M., Presynaptic GABAB receptors on rat hippocampal neurons, in GABAB Receptors in Mammalian Function, Bowery, N.G., Bittiger, H. and Olpe, H.?R., eds., Wiley, pp. 207?221, 1990 (invited). Teyler, T., Aroniadou, V., Berry, R.L., Borroni, A., DiScenna, P., Grover, L. and Lambert, N., LTP in neocortex, Seminars in Neuroscience, Gustafsson, B. and Wigstrom, H. eds., Vol. 2, pp. 365-379, 1990 (invited). Lambert, N.A., Harrison, N.L., Kerr, D.I.B., Ong, J., Prager, R.H. and Teyler, T.J. Blockade of the late IPSP in rat CA1 hippocampal neurons by 2?hydroxy?saclofen. Neurosci. Lett., 107:125?127, 1989. Harrison, N.L. and Lambert, N.A., Modification of GABAA receptor function by an analog of cyclic AMP. Neurosci. Lett., 105:137?142, 1989.

Education and Training

Duke University, Durham, NC:
Post Doctoral Training, 1991-1994.

Kent State University, Kent, OH:
Ph.D.- Biomedical Sciences (Neuroscience), 1991.

Youngstown State University,Youngstown, OH:
B.S. - Combined Sciences, 1990

Research Experience and Academic Appointments

2001-present - Associate Professor, Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia.

2000-2004 - Summer Faculty, Marine Biological Laboratory, Woods Hole, Massachusetts.

1996-2004 - Research Physiologist, Veterans Affairs Medical Center, Augusta, Georgia.

1996-2001 - Assistant Professor, Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia.

1995 - Research Assistant Professor, Department of Pharmacology, Duke University Medical Center, Durham, North Carolina.

1991-1994 - Research Associate (postdoctoral fellow), Department of Pharmacology, Duke University Medical Center, Durham, North Carolina.

Research Physiologist, Veterans Affairs Medical Center, Durham, North Carolina.

1987-91 - Graduate Assistant, Department of Neurobiology, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio (advisor: T.J. Teyler).

1987 - Summer Research Fellow, Laboratory of Neurophysiology, NINCDS, The National Institutes of Health, Bethesda, Maryland.

Teaching Experience

Northeastern Ohio Universities College of Medicine/Kent State University:

Teaching Assistant, Molecular Pathology (Biochemistry), and Physiology
Teaching Assistant, Department of Neurobiology (taught medical and graduate neuroanatomy and neurophysiology)

Medical College of Georgia:

Molecular Pharmacology (PHM 801), 1996-present
Human Pharmacology (PHM551), 1996-present
Introduction to Pharmacology (PHM301), 1996-present
Small Group Elective, Molecular Targets of Drug Action (PHM), 1997
Core Curriculum Functional Systems Biology (SGS 804), 1997-present (Neuroscience Block Co-director, 1999, Director, 2001-present; Pharmacology Unit Director, 2000).
Core Curriculum Functional Cellular Biology (SGS), 1997-present
Medical Neuroscience (ITD), 1997-present

Marine Biological Laboratory, Woods Hole, MA:

Neurobiology Summer Course, 2000-2004.

Society Memberships and Committees

Peer-review Activity:

Member, Panel for Instrument Development for Biological Research (IDBR), Division of Biological Infrastructure, Bio Directorate, National Science Foundation, 11/12/96-11/12/99
Ad hoc reviewer, MCDN?3 Study Section, NINDS, NIH
Ad hoc reviewer, Veterans Administration intramural funding program (Merit Review)
Grant reviewer, Paralyzed Veterans of America
Reviewer for: Neuron, Journal of Physiology, Journal of Neurophysiology, Journal of Neuroscience, Neuropharmacology, Neuroscience, Neuroscience Letters, Hippocampus, Brain Research, Biophysical Journal, British Journal of Pharmacology, General Pharmacology, European Journal of Neuroscience, Journal of Pharmacology and Experimental Therapeutics

Institutional Service:

President, School of Medicine Faculty Senate, 2001-2002.
Committee on Medical Education, Department of Pharmacology and Toxicology, 1996-2000.
Faculty Search Committees, Department Pharmacology and Toxicology, 1996-2002.
Institutional Animal Care and Use Committee (Augusta VAMC), 1996-present.
Senator, Graduate Faculty Senate, 1997-1998.
Faculty Grievance Committee, 1998-2001.
Secretary, Faculty Senate Executive Committee, 1998-1999.
Vice President, Faculty Senate Executive Committee, 1999-2000.
President-elect, Faculty Senate Executive Committee, 2000-2001.
Research and Development Committee (Augusta VAMC), 1997-present.
Graduate Student Research Day Committee, 1999-2000.
Graduate Education Committee, Department of Pharmacology and Toxicology, 1999.
Medical Student Interviewer for Admissions, 1999-present.
Faculty Recognition Committee (Chairman-1999), 1999-2000.
Director, Graduate Program, Department of Pharmacology and Toxicology, 1999-present.
Neuroscience Steering Committee, 1999.
MD/PhD Advisory Committee, 2000-present.
Recruitment and Admissions Committee, School of Graduate Studies, 1999-present.
Curriculum and Advancement Committee, School of Graduate Studies, 2001-present.
Chair, Cell Signaling Eminent Scholar Search Committee, 2002.