Chic chorioallantoic membrane (CAM) angiogenesis assay, microvascular corrosion casting, peptide synthesis, HPLC (analytical), endothelial cell cultures, cell proliferation assays, matrigel angiogenesis assay of in vitro tube formation, zymography, SDS-PAGE, western blotting, immunocytochemistry, scanning electron microscopy, transmission electron microscopy, computers, electrophysiology (whole cell, inside-out patch clamping), smooth muscle tension studies, RT-PCR, various biochemical assays.
Program Project Grant (DK38226), PI: J.H. Capdevila, 7/04 - 6/2007
Project (P.I: John D. Imig)- $900,040
Role of Eicosanoids in Renal Function.
The major goals of this study is to define the role of normally produced eicosanoids and their mimetics in renal function.
Role: Collaborator - 25% Effort
R01 04/01/2005 to 03/31/2009 50% effort
NIH/NHLBI - $971,000
Protection of asthma exacerbation by estrogen.
Role: Principal Investigator
MCG Combined Intramural Grant Program, 9/2003-8/2004
Molecular basis of estrogen action in airway smooth muscle - $17,000
The object of the project was to define molecular mechanism of estrogen action in airway smooth muscle cells.
Role: Principal Investigator
RG-170-N 7/2003-6/2005 50% Effort
American Lung Association Research Grant* $70,000
Molecular basis of estrogen action in airway smooth muscle
The object of the project is to define molecular mechanism of estrogen action in airway smooth muscle cells.
Role: Principal Investigator
*Highest rated application from the state of Georgia for 2003.
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1994 Travel Award to NATO ASI on "Vascular Endothelium: Reponses to Injury", Crete, Greece.
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1994 Travel Award to NATO Summer School on "Signal transduction mechanisms", Spetses, Greece.
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1995 Travel Award to NATO ASI on "Angiogenesis: Models, Modulators and Clinical Applications", Porto Carras, Greece.
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1996 Travel Award to NATO ASI on "Vascular Endothelium: Pharmacologic and Genetic Manipulations", Crete, Greece.
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1997 Travel Award to NATO ASI on "Angiogenesis: Models and modulators", Rhodes, Greece.
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1995-1997 Pre-Doctoral Fellowship, Human Capital Mobility Project, European Economic Community.
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2000 Travel Award to NATO ASI on "Vascular Endothelium: Mechanism of Cell Signaling", Crete, Greece.
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2000 NAVBO Travel Award to the International Vascular Biology Meeting in Geneva.
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2003 AHA Travel Award to the AHA meeting, Orlando, FL.
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2004 First place award at the Panhellenic Congress on Lung Disease Meeting .
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2005 Travel award to the 3rd ERS Lung Science Conference, Taormina, Italy.
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2005 Invited Speaker for the annual ERS meeting in Copenhagen, Denmark.
Refereed Papers
Konerding, M.A., Fait, E., Dimitropoulou, C., Malkusch, W., Ferri, C, Giavazzi, R., Coltrini, D. and Presta, M. Impact of Basic Fibroblast Growth Factor (bFGF) on Tumor Microvascular Architecture: A 3D Morphometric Study. Am J Pathol., 152: 1607-15, 1998.
Dimitropoulou C., Malkusch W., Fait E., Maragoudakis,M. E. and Konerding M.A. The Vascular Architecture of the Chick Chorioallantoic Membrane: Sequencial Quantitative Evaluation Using Corrosion Casting. Angiogenesis 2: 255-263, 1998.
Dimitropoulou, C., Maragoudakis, M.E., Konerding, M.A. Effects of thombin and D609 on the chick chorioallantoic membrane: Quantitative microvascular corrosion casting study. Gen. Pharm. 35, 241-7, 2000.
Dimitropoulou, C., White, R.E., Fuchs, L.C., Zhang H., Catravas, J.D., Carrier G.O. Angiotensin II relaxes microvessels via the AT(2) receptor and Ca2+-activated K+ (BKCa) channels. Hypertension 37: 301-307, 2001.
Miller, A.W., Dimitropoulou, C., Han, G., Carrier, G.O., White, R.E. and Busija, D.W. Epoxyeicosatrienoic acid induced relaxation is impaired in insulin resistance. Amer. J. Physiol. 281, H1524-H1531, 2001.
R.E. White, G. Han, M. Maunz, C. Dimitropoulou, A.M. El Mowafy, R.S. Barlow, J. D. Catravas, C. Snead, G. O. Carrier, S. Zhu, X. Yu. Endothelium independent effect of estrogen on Ca 2+ activated K+ channels in human coronary artery smooth muscle cells. Cardiovasc. Res. 53: 650-661, 2002.
Dimitropoulou, C., Han G., White, R.E., Carrier, G.O., and Miller A. Potassium (BK(Ca)) currents are reduced in microvascular smooth muscle cells from insulin-resistant rats. Amer. J. Physiol. 282:H908-917, 2003.
Venema, R.C., Venema, V.J., Ju, H., Harris, M.B., Snead, C., Dimitropoulou, C., Catravas, J.D. Novel complexes of guanylate cyclase with heat shock protein 90 and nitric oxide synthases enhance cyclase activity. Amer. J.Physiol. 285: H669-H678, 2003.
Fait, E., Malkusch, W., Gnoth, S.H., Dimitropoulou, C., Kirkpatrick, C.J., Junginger, T. and Konerding, M.A. Microvascular patterns of the human large intestine: morphometric studies of the inter-capillary distances, interbranching distances and branching angles in corrosion casts. Scann. Microsc. 11:xxx-xxx (in press; galley proofs available).
Dimitropoulou, C., White, R.E., Ownby, D.R. and Catravas, J.D. Estrogen reduces carbachol constriction of airway smooth muscle by stimulation BKCa channels AJRCMB, 2005 Mar;32(3):239-47.
White, R.E., Han, G., Dimitropoulou, C, Zhu, S., Miyake, K., Fulton, D., Dave, S., Barman, S.A. Estrogen-induced contraction of coronary arteries is mediated by superoxide generated in vascular smooth muscle. Amer J. Physiol, Heart Circ Physiol. 2005 Oct;289(4):H1468-75.
G. Yetik-Anacak, T. Xia, C. Dimitropoulou, R. Venema, J. Catravas. Effects of HSP90 binding inhibitors on sGC-mediated vascular relaxation. Amer J. Physiol:Heart Circ Physiol. 2006 Jul;291(1):H260-8.
Submitted Papers
Han, G., Fulton, D., Dimitropoulou, C., Zhu, S. and White, R.E. Estrogen-induced contraction of coronary arteries mediated by Type 1 (n)NOS and superoxide. AJP, Heart and Circulatory Physiology.
Ranta, P.M., Dimitropoulou, C., Ford, J., Young, P. and Ownby, D.R. Identification and characterization of cross-reactive poinsettia (Euphorbia pulcherrima) and natural rubber latex (Hevea brasiliensis) allergens An. Allergy..
Imig, J.D., Dimitropoulou, C., Falck, J.R. and White, R.E. Afferent arteriolar dilation to 11,12-EET analogs Involves increases in cAMP levels, PP2A activity and activation of Ca2+-activated K+ (BKCa) channels. Journal of Biological Chemistry.
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