|
2 |
Causes
disease in healthy adults. Transmitted
by percutaneous injury, ingestion, or
mucous membrane exposure.
This includes
human blood, body fluids, unfixed human
tissue, or uncharacterized human cells. |
BSL-1
Practices plus: Limited access,
Biohazard warning signs, "Sharps"
precautions, Biosafety SOP |
Biological Safety Cabinets (BSC’s) or
other physical containment devices for
all procedures with agents that could
cause splashes or aerosols of infectious
materials. PPE includes laboratory
coats, gloves, face protection as needed |
BSL-1
Requirements plus: Autoclave available
if needed |
|
3 |
Causes
serious disease in healthy adults.
Transmitted by aerosols. |
BSL-2
Practices plus: Controlled access to
lab; Decontamination of all waste.
Decontamination of lab clothing before
laundering. Baseline serum as required |
BCSs
or other physical containment devices
used for all open manipulations of
agents. PPE includes protective lab
clothing, gloves, respiratory protection
as needed |
BSL-2
Requirements plus: Physical separation
from access corridors. Self-closing,
double-door access. Exhausted air not
recirculated. Negative airflow into
laboratory |
|
4 |
Dangerous/exotic agents which pose high
risk of life-threatening disease.
Threat
to lab personnel by aerosol transmission
or unknown transmission risk |
BSL-3
Practices plus: Clothing change before
entering; shower before exiting. All
material leaving lab must be
decontaminated. |
All
procedures conducted in BSCs with
full-body, air-supplied, positive
pressure suit |
BSL-3
Requirements plus: Separate building or
isolated zone. Dedicated air supply,
exhaust, vacuum, and waste
decontamination systems |