Human and Animal Biosafety Level 3 Pathogens
Human and Animal Biosafety Level 3 Pathogens cause
serious or potentially lethal disease in humans and
present the potential for aerosol transmission.
BSL = Biological Safety Level
ABSL = Animal Biological Safety Level
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|
AGENT |
BSL |
ABSL |
COMMENTS |
|
BACTERIAL AGENTS: |
|
BACILLUS ANTHRACIS |
2 |
3 |
Animal biosafety level 2 practices are
adequate for experimentally infected
rodents. Biosafety level 3 practices are
recommended for work involving
production volumes, concentrations of
cultures, or activities with high
potential for aerosol production.
Vaccination is recommended in these
facilities for all persons working in
the same room with the cultures, and
persons working with infected animals.
|
|
BARTONELLA SP 3 3 |
3 |
3 |
|
|
BRUCELLA SP 3 3 |
3 |
3 |
Biosafety level 2 practices are
recommended for activities involving
clinical material. Biosafety level 3
practices are recommended for work with
cultures and Animal Biosafety Level 3
practices are recommended for
experimental animal studies. |
|
CHLAMYDIA PSITTACI |
2 |
3 |
Biosafety level 2 practices are
recommended for necropsies of infected
birds and diagnostic examination of
tissues or cultures. Consult CDC Guide
for more details. Biosafety level 3
practices indicated when a high
potential for aerosolization is present
|
|
CHLAMYDIA TRACHOMATIS |
3 |
3 |
|
|
FRANCISELLA TULARENSIS |
3 |
3 |
Biosafety level 2 practices are
recommended for activities with clinical
material potentially containing
Francisella. Level 3 practices are
indicated for cultures or experimental
animal work. |
|
MYCOBACTERIUM BOVIS |
3 |
3 |
Level 2 practices are indicated for
preparing acid-fast smears and for
culturing clinical specimens, provided
that manipulation of such cultures is
conducted in a Class I or Class II
biological safety cabinet. Level 3
practices are indicated for propagation
of cultures and for work with
experimental animals |
|
MYCOBACTERIUM TUBERCULOSIS |
3 |
3 |
See Mycobacterium bovis, above. |
|
PSEUDOMAS MALLEI |
3 |
3 |
|
|
YERSINIA PESTIS |
3 |
3 |
Immunization is recommended for people
working with cultures or with infected
rodents. Necropsies should be conducted
in a hood. Level 3 practices are
indicated when working with production
quantities of cultures or when there is
a potential for aerosol production. |
|
FUNGAL AGENT: |
|
BLASTOMYCES DERMATITIDIS |
3 |
2 |
Level 2 practices are indicated for
activities with clinical materials,
animal tissues, and infected animals.
Level 3 practices are indicated for
processing mold cultures, soil, and
environmental materials likely to
contain infectious conidia. |
|
COCCIDIOIDES IMMITIS |
3 |
2 |
Animal Biosafety level 2 parctices are
indicated when the route of challenge is
parenteral. Level 2 practices are
indicated for clinical specimens and
animals tissues. Level 3 practices are
indicated for mold cultures or infected
environmental materials. |
|
HISTOPLASMA CAPSULATUM |
3 |
2 |
Level 2 practices may be used to handle
clinical specimens, animal tissues, or
for experimental animal studies when the
route of challenge is parenteral. Level
3 practices are indicated for mold
cultures, soil, or environmental
materials likely to contain infectious
conidia. |
|
RICKETTSIAL AGENTS: |
|
COXIELLA BURNETII |
3 |
3 |
Level 2 practices may be used for non-propagative
studies such as serological examinations
and staining of impression smears. Level
3 practices are recommended for
innoculation, incubation, and harvesting
of eggs or cultures, necropsy of
infected animals, and manipulation of
infected tissues. Experimentally
infected rodents should be maintained
under Animal Biosafety Level 3. |
|
RICKETTSIA CANADA |
3 |
2 |
Biosafety level 2 procedures are
indicated for non_propagative laboratory
procedures such as serological
procedures and staining of impression
smears. Biosafety level 3 practices are
indicated for all other manipulations of
infectious material, including necropsy
of infected animals and trituration of
their tissues, and for inoculation,
incubation, and harvesting of eggs and
tissues. Animal Biosafety level 2
practices may be used to house
experimentally infected animals except
for arthropods and flying squirrels
(which require Animal Biosafety Level
3). |
|
RICKETTSIA PROWAZEKII |
3 |
2 |
See Rickettsia canada |
|
RICKETTSIA RICKETTSII |
3 |
2 |
Biosafety Level 2 practices apre used
for all non-propagative procedures, such
as serological work and staining
impression smears. Biosafety level 3
practices are used for all other
manipulations of known or potentially
infected material, such as cultures,
necropsy of infected animals, and
innoculation and harvesting of eggs.
Infected animals may be housed in Animal
Biosafety level 2 facilities provided
that necropsies and any subsequent
manipulation of tissues is conducted at
Biosafety Level 3. Workers must report
any febrile illness promptly. |
|
RICKETTSIA TSUTSUGAMUSHI |
3 |
2 |
See Rickettsia canada. |
|
RICKETTSIA TYPHI (R.MOOSERI) |
3 |
2 |
See Rickettsia canada. |
|
SPOTTED FEVER GROUP - OTHER |
3 |
2 |
See Rickettsia canada. |
|
VIRAL AGENTS: |
|
ARBOVIRUSES-CERTAIN |
3 |
3 |
|
|
ARENAVIRUSES-CERTAIN |
3 |
3 |
|
|
DENGUE VIRUS |
2 |
3 |
|
|
HERPESVIRUS ATELES |
3 |
3 |
|
|
HERPESVIRUS SAIMIRI |
3 |
3 |
|
|
INFLUENZA VIRUS VIRULENT AVIAN |
3 |
3 |
|
|
LYMPHOCYTIC CHORIOMENINGITIS |
3 |
3 |
|
|
POXVIRUS ALASTRIM |
3 |
3 |
|
|
POXVIRUS MONKEY POX |
3 |
3 |
|
|
POXVIRUS SMALLPOX |
3 |
3 |
|
|
RABIES VIRUS |
3 |
3 |
|
|
VACCINA VIRUS |
2 |
3 |
|
|
VENEZUELAN EQUINE ENCEPHALITIS |
3 |
3 |
|
|
VESICULAR STOMATITIS VIRUS |
3 |
3 |
Biosafety level 3 practices are
indicated for manipulation of infected
tissues, and for culture of virulent
isolates from naturally or
experimentally infected livestock. |
|
WOOLY MONKEY FIBROSARCOMA |
3 |
3 |
|
|
YABA VIRUS |
3 |
3 |
|
|
YELLOW FEVER VIRUS EXCEPT 17D |
3 |
3 |
|