Ascites Fluid Collection

PHS Policy requires the following information if experiments involve the mouse ascites method to produce antibodies:

• A description of the ascites production and collection. Include frequencies of collection, method of collection and total number of abdominal taps;


• a scientific justification for using the ascites method


• Alternative methods you have considered that would avoid or minimize distress, discomfort and pain (including in vitro methods) and your reasons for concluding that those methods are unsuitable. Information about alternative methods are available in the LAS office.


• Accumulation of fluid in the peritoneal cavity causes abdominal distention, discomfort and in extreme cases may even cause respiratory distress. The investigator must tap the excess fluid frequently enough to minimize this distention.


• The animals must be observed at least three times per week for the first week after injection of the tumor cells. Afterwards, they must be observed daily by the investigators staff. The ascites must be drained before it becomes excessive and before respiratory distress is evident.
  

Ascites fluid may be collected with an 18-22 gauge hypodermic needle. Anesthesia is not normally required if those performing the collection are experienced. The animals should be anesthetized if new personnel are being trained.