Protocols

Biochemistry

• Elisa assay
• Immunoisolation of synaptic vesicles
• Synaptosome preparation

Cell Biology

• Brain cell culture
  • Astroglial cell culture
  • Axon outgrowth assay by explant culture
  • Cell culture N19 & N20
  • Cerebellar granule neuron culture and migration recording
  • Cortical neuron culture
  • Glial cell cultures
  • Hippocampal cell culture
  • Oli-neu culture
  • Xenopus neurite culture
     
• Muscle cell culture
  • C2C12 cell culture
  • Chick embryo muscle culture
  • Primary rat muscle culture
     

Electron Microscope

• EM protocol for mouse diaphragm

Histology

• BrdU labeling
• Immunochemistry for neuron culture
• In Situ( E18 diaphragm mouse)
• Whole mount staining (mouse diaphragm)

Molecular Biology

• DNARNA
   
  • Cell transfection with calcium phosphate
  • Electrical stimulation of embrionic rat primary skeletal mus
  • Generation of recombinant adenoviruses
  • Luciferase assay
  • Retrovirus transient transfection of 293GPG packaging cells
  • RT-PCR
  • Subcloning
  • Transformation
     
• Protein
   
  • Beta galactosidase assay
  • Biotinylation of cell surface proteins
  • CAT assay
  • GST- fusion protein purification and pull down assay
  • Immunoprecipitation
  • PAK1 kinase assay
  • PLC assay
  • Recepter endocytosis
  • RHO GAP assay
  • Western blotting
  • Yeast two-hybrid

Copyright 2007
Medical College of Georgia
All rights reserved.

Institute of Molecular Medicine and Genetics
Medical College of Georgia
Please e-mail comments, suggestions or questions to:
Laura Hutcheson, ljhutche@mcg.edu

07/23/2007