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Flow Cytometry Core Laboratory
Fluorochrome Options

(see User Guidelines regarding Multicolor fluorochrome experiments)

Fluorochromes excited by 488nm detectable by FL1 (530±15 or 20nm)

  • FITC
  • GFP
  • Alexa 488
  • Cy2
  • Fluo-3
  • Calcein
  • CFSE
  • FMLP
  • DCFH
  • TOTO-1
  • TO-PRO-1
  • BODIPY-FL
  • YOYO-1
  • SYTOX Green
  • DHR 123

back to: Flow Cytometry Core Laboratory Instrumentation

Fluorochromes excited by 488nm detectable by FL2 (585±21nm, 580± 15nm and 575±13nm)  

  • PE
  • Cy3
  • PI 

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Fluorochromes excited by 488nm detectable by FL3 670+ and 675+nm  (FACSCalibur)

  • PerCP
  • PE-Cy5 (CyChrome, Tricolor, Quantum Red)
  • PE-Cy7
  • PerCP-Cy5.5 (TruRed)
  • PI

back to: Flow Cytometry Core Laboratory Instrumentation

Fluorochromes excited by 633nm detectable by FL4 660 ±8 or 10nm (FACSCalibur)

  • APC
  • Cy5
  • Alexa Fluor 633

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Fluorochromes excited by 647nm detectable by FL6 on DakoCytomation MoFlo

  • APC

  • Cy5

  • Alexa Fluor 647

 

Fluorochromes excited by 488nm detectable by FL4-670±15 on DakoCytomation MoFlo

  • PerCP
  • PE-Cy5 (CyChrome, Tricolor, Quantum Red)
  • PE-Cy7
  • PerCP-5.5 (TruRed)
  • PI

back to: Flow Cytometry Core Laboratory Instrumentation
 
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Please email comments, suggestions or questions to:
Abhijit Afzalpurkar, aafzalpurkar@mcg.edu
December 05, 2006