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Flow Cytometry Core Laboratory
Instrumentation

Three- and four-color Becton Dickinson FACS Calibur flow cytometers are available for biological cell analysis. These instruments collect information on light scatter (indicating approximate cell size and complexity of interior cell structure) and up to three or four colors of fluorescence emission. These machines can be operated a rates of up to several thousand cells per second. We also have 2 cell sorters, one, the MoFlo, that can perform 5+ color analysis (for more information of multicolor analysis see User Guidelines).

Analysers
Becton Dickinson FACSCalibur Flow Cytometers
4-color analyser
...equipped with 2 lasers 1° laser – 15mW 488nm air-cooled argon-ion laser  
2° laser – 635nm red diode laser 
...and 4 fluoresence detectors FL1 – 530 ± 15nm (FL1 fluorochrome options)
FL2 – 585 ± 21nm (FL2 fluorochrome options)
FL3 – 670+nm (FACSCalibur FL3 fluorochrome options)
FL4 – 661 ± 8nm (BD FL4 fluorochrome options)
3-color analyzer
...equipped with 1 laser 15mW 488nm air-cooled argon- ion laser
...and 3 fluorescence detectors FL1 – 530 ± 15nm  (FL1 fluorochrome options)
FL2 – 585 ± 21nm (FL2 fluorochrome options)
FL3 – 670+nm   (BD FL3 fluorochrome options)
Cell Sorters
DakoCytomation MoFlo (sorts up 4 separate cell populations at speeds of up to 70,000 cells/second)
 9-color sorter
(see Facility User Guidelines regarding Multicolor fluorochrome experiments)
...equipped with 3 lasers 1° laser – Spectra-Physics 177G 488nm air-cooled argon- ion laser
2° laser – Coherent Innova 90C argon UV laser
  3° laser – Coherent  Innova 70 argon/krypton  647nm red diode laser 
...and 9 fluorescence detectors From 1° laser
FL1 – 530 ± 20nm (FL1 fluorochrome options)
FL2 – 580 ± 15nm (FL2 fluorochrome options)
  FL3 – 630 ± 15nm (MoFlo FL3 fluorochrome options – PE-Texas Red and PI)
  FL4 670±15nm (MoFlo FL4 fluorochrome options – PerCP and PE-Cy5)
  FL5 – 740+nm ( MoFlo FL5 fluorochrome – PE-Cy7)
  From 2°  laser  (UV excitation for Calcium Flux with Indo-1, Hoechst, DAPI and other UV excited fluorophores)
FL6 – 405 ± 15nm or 450 ± 33nm
FL7 – 450 ± 33nm or 485 ± 13nm or 630 ± 15nm
From 3° laser
FL8 – 670 ± 10nm (MoFlo FL8 fluorochrome options)
  FL9 – 740+nm ( MoFlo FL9 fluorochrome option - APC-Cy7)
   
   
   
   
   
   
 
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Please email comments, suggestions or questions to:
Abhijit Afzalpurkar, aafzalpurkar@mcg.edu
December 05, 2006